基质金属蛋白酶10(MMP10)重组蛋白

YB098Ra011
Recombinant Matrix Metalloproteinase 10 (MMP10)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 基质金属蛋白酶10(MMP10)重组蛋白9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Phe99~Cys476 (Accession # P09238),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.5
 基质金属蛋白酶10(MMP10)重组蛋白Predicted Molecular Mass: 44.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-FS SFPGMPKWRK THLTYRIVNY TPDLPRDAVD SAIEKALKVW
EEVTPLTFSR LYEGEADIMI SFAVKEHGDF YSFDGPGHSL AHAYPPGPGL YGDIHFDDDE
KWTEDASGTN LFLVAAHELG HSLGLFHSAN TEALMYPLYN SFTELAQFRL SQDDVNGIQS
 基质金属蛋白酶10(MMP10)重组蛋白LYGPPPASTE EPLVPTKSVP SGSEMPAKCD PALSFDAIST LRGEYLFFKD RYFWRRSHWN
PEPEFHLISA FWPSLPSYLD AAYEVNSRDT VFIFKGNEFW AIRGNEVQAG YPRGIHTLGF
PPTIRKIDAA VSDKEKKKTY FFAADKYWRF DENSQSMEQG FPRLIADDFP GVEPKVDAVL
QAFGFFYFFS GSSQFEFDPN ARMVTHILKS NSWLHC
[ REFERENCES ]
1. Bertini I., et al. (2004) J. Mol. Biol. 336:707-716. 2. Wertz K., et al. (2004) Free Radic. Biol. Med. 37:654-670. 3. Schnabl B., et al. (2005) Biochem. Biophys. Res. Commun. 335:87-96. 4. Aung P.P., et al. (2006) Oncogene 25:2546-2557.